LOCUS NC_008048 3345170 bp DNA circular BCT 30-MAY-2006
DEFINITION Sphingopyxis alaskensis RB2256, complete genome.
ACCESSION NC_008048
VERSION NC_008048.1 GI:103485498
KEYWORDS .
SOURCE Sphingopyxis alaskensis RB2256
ORGANISM Sphingopyxis alaskensis RB2256
Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales;
Sphingomonadaceae; Sphingopyxis.
REFERENCE 1 (bases 1 to 3345170)
AUTHORS Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina
del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S.,
Brettin,T., Bruce,D., Han,C., Tapia,R., Munk,A.C., Chertkov,O.,
Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N.,
Kim,E., Cavicchioli,R., Robb,F., Ertan,H., Schut,F., Ting,L.M. and
Richardson,P.
CONSRTM US DOE Joint Genome Institute
TITLE Complete sequence of chromosome of Sphingopyxis alaskensis RB2256
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 3345170)
CONSRTM NCBI Genome Project
TITLE Direct Submission
JOURNAL Submitted (30-MAY-2006) National Center for Biotechnology
Information, NIH, Bethesda, MD 20894, USA
REFERENCE 3 (bases 1 to 3345170)
AUTHORS Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina
del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S.,
Brettin,T., Bruce,D., Han,C., Tapia,R., Munk,A.C., Chertkov,O.,
Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N.,
Kim,E., Cavicchioli,R., Robb,F., Ertan,H., Schut,F., Ting,L.M. and
Richardson,P.
CONSRTM US DOE Joint Genome Institute
TITLE Direct Submission
JOURNAL Submitted (20-APR-2006) US DOE Joint Genome Institute, 2800
Mitchell Drive B100, Walnut Creek, CA 94598-1698, USA
COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final
NCBI review. The reference sequence was derived from CP000356.
URL -- http://www.jgi.doe.gov
JGI Project ID: 3634495
Source DNA and bacteria available from Rick Cavicchioli
(rcavicchioli@unsw.edu.au)
Contacts: Rick Cavicchioli (rcavicchioli@unsw.edu.au)
Paul Richardson (microbes@cuba.jgi-psf.org)
Quality assurance done by JGI-Stanford
Annotation done by JGI-ORNL and JGI-PGF
Finishing done by JGI-LLNL
Finished microbial genomes have been curated to close all gaps with
greater than 98% coverage of at least two independent clones. Each
base pair has a minimum q (quality) value of 30 and the total error
rate is less than one per 50000.
The JGI and collaborators endorse the principles for the
distribution and use of large scale sequencing data adopted by the
larger genome sequencing community and urge users of this data to
follow them. it is our intention to publish the work of this
project in a timely fashion and we welcome collaborative
interaction on the project and analysis.
(http://www.genome.gov/page.cfm?pageID=10506376)
Additional Notes:
Sphingopyxis alaskensis RB2256 appears to have about a 28.5kb
extrachromosomal piece of DNA with significantly lower coverage
than the chromosome. It appears to be of F plasmid origin because
of the presence of traA gene, a conjugal transfer protein. It can
be integrated into the genome between 856688 and 859197, excising a
piece of chromosomal DNA 2517 bp long. Perhaps a fraction of the
population has this 28.5 kb fragment integrated. Within 2 kb of
the excision point is an IS21 element with ORF1 and ORF2 of
transposase and perfect inverted repeats. However, we have been
unsuccessful in circularizing the 28.5 kb fragment with pcr,
perhaps because of the low representation in the genome.
COMPLETENESS: full length.