LOCUS NC_008255 4433218 bp DNA circular BCT 25-JUL-2006
DEFINITION Cytophaga hutchinsonii ATCC 33406, complete genome.
ACCESSION NC_008255
VERSION NC_008255.1 GI:110636427
KEYWORDS .
SOURCE Cytophaga hutchinsonii ATCC 33406
ORGANISM Cytophaga hutchinsonii ATCC 33406
Bacteria; Bacteroidetes; Sphingobacteria; Sphingobacteriales;
Flexibacteraceae; Cytophaga.
REFERENCE 1 (bases 1 to 4433218)
AUTHORS Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina
del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S.,
Brettin,T., Bruce,D., Challacombe,J.F., Chertkov,O., Han,S.,
Tapia,R., McBride,M.J., Misra,M., Myers,G.L., Thayer,N.N.,
Thompson,L.S., Xie,G., Gilna,P., Schmutz,J., Larimer,F., Land,M.,
Hauser,L., Kyrpides,N., Lykidis,A., Ivanova,N., Mavrommatis,K.,
Rubin,E. and Richardson,P.
CONSRTM US DOE Joint Genome Institute
TITLE Complete sequence of Cytophaga hutchinsonii ATCC 33406
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 4433218)
AUTHORS .
CONSRTM NCBI Genome Project
TITLE Direct Submission
JOURNAL Submitted (24-JUL-2006) National Center for Biotechnology
Information, NIH, Bethesda, MD 20894, USA
REFERENCE 3 (bases 1 to 4433218)
AUTHORS Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina
del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S.,
Brettin,T., Bruce,D., Challacombe,J.F., Chertkov,O., Han,S.,
Tapia,R., McBride,M.J., Misra,M., Myers,G.L., Thayer,N.N.,
Thompson,L.S., Xie,G., Gilna,P., Schmutz,J., Larimer,F., Land,M.,
Hauser,L., Kyrpides,N., Lykidis,A., Ivanova,N., Mavrommatis,K.,
Rubin,E. and Richardson,P.
CONSRTM US DOE Joint Genome Institute
TITLE Direct Submission
JOURNAL Submitted (02-JUN-2006) US DOE Joint Genome Institute, 2800
Mitchell Drive B100, Walnut Creek, CA 94598-1698, USA
COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final
NCBI review. The reference sequence was derived from CP000383.
URL -- http://www.jgi.doe.gov
JGI Project ID: 2351473
Source DNA and bacteria available from Mark McBride
Contacts: Mark McBride (mcbride@uwm.edu)
Paul Richardson (microbes@cuba.jgi-psf.org)
Plasmid and fosmid libraries were prepared at the Joint Genome
Institute (JGI) Production Genomics Facility (JGI-PGF) in Walnut
Creek, CA. Shotgun sequencing was performed at the JGI-PGF to a
coverage of 15x. Large (40 kb), medium (8 kb) and small (3 kb)
insert random libraries were partially sequenced with average
success rate of 90% and average high-quality read lengths of 634
nucleotides. Finishing was performed at JGI-LANL starting with 29
contigs and 2 scaffolds. Repetitive regions were identified,
assembled and finished by manually checking paired reads close to
each repeat in the assembly with consed and then making a
subassembly for each repetitive region. 27 gaps were closed with
primer walks and 2 by PCR. Gene predictions were obtained using
Glimmer and tRNAs were identified using tRNAScan-SE. Basic analysis
of the gene predictions was performed by comparing coding sequences
against the PFam, BLOCKS and Prodom databases. Gene definitions and
functional classes were added manually by a team of annotators at
JGI-LANL, using BLAST results in addition to information from the
basic analysis. A total of 3793 features have been annotated on the
sequence record.
The JGI and collaborators endorse the principles for the
distribution and use of large scale sequencing data adopted by the
larger genome sequencing community and urge users of this data to
follow them. it is our intention to publish the work of this
project in a timely fashion and we welcome collaborative
interaction on the project and analysis.
(http://www.genome.gov/page.cfm?pageID=10506376).
COMPLETENESS: full length.